Rauvala H
J Cell Biol. 1984 Mar;98(3):1010-6. doi: 10.1083/jcb.98.3.1010.
Neurite outgrowth of C 1300 neuroblastoma cells, which were dispersed from adherent cultures or grown in suspension, was studied on different protein-coated surfaces. Of 29 different surface structures studied, including surfaces treated with various fibronectins, lectins, glycosidases, or glycosyltransferases capable of stimulating fibroblast spreading, only the surfaces coated with plasma fibronectin or with a protein mixture secreted by C6 glioma cells displayed an extensive activity in the sprouting assay. Neurite outgrowth was inhibited by brain gangliosides and by colominic acid (a sialic acid polymer). A 50% inhibition of neurite outgrowth of N18 neuroblasts induced by the glioma cell proteins was observed at the following approximate concentration: 100 microM (0.2 mg/ml) GD1A ganglioside, 20 microM (0.04 mg/ml) GT1B ganglioside, and 5 mg/ml colominic acid. Specificity of inhibition was suggested by the finding that a few polyanionic substances tested were not inhibitory in the sprouting assay, and that the type of gangliosides inhibiting sprouting were found to be major sialoglycolipids of the neuroblasts. A hypothesis is discussed, according to which neurite outgrowth of neuroblasts is stimulated by adhesion involving interactions of the adhesion-mediating protein with cell surface carbohydrates characteristic of brain gangliosides.
研究了从贴壁培养物中分散或悬浮培养的C1300神经母细胞瘤细胞在不同蛋白质包被表面上的神经突生长情况。在研究的29种不同表面结构中，包括用各种纤连蛋白、凝集素、糖苷酶或能够刺激成纤维细胞铺展的糖基转移酶处理过的表面，只有用血浆纤连蛋白或C6胶质瘤细胞分泌的蛋白质混合物包被的表面在发芽试验中表现出广泛的活性。神经突生长受到脑神经节苷脂和结肠酸（一种唾液酸聚合物）的抑制。在以下近似浓度下观察到胶质瘤细胞蛋白诱导的N18神经母细胞神经突生长受到50%的抑制：100微摩尔（0.2毫克/毫升）GD1A神经节苷脂、20微摩尔（0.04毫克/毫升）GT1B神经节苷脂和5毫克/毫升结肠酸。测试的几种聚阴离子物质在发芽试验中无抑制作用，以及发现抑制发芽的神经节苷脂类型是神经母细胞的主要唾液酸糖脂，这些发现表明了抑制的特异性。讨论了一种假说，即神经母细胞的神经突生长是由涉及粘附介导蛋白与脑神经节苷脂特征性细胞表面碳水化合物相互作用的粘附所刺激的。
