在分化过程中，Friend细胞中的DNA聚合酶活性。

DNA polymerase activities in Friend cells during the differentiation process.

作者信息

Lacatena R M, Busiello V, di Girolamo A, di Girolamo M

出版信息

Cell Differ. 1981 Mar;10(2):109-16. doi: 10.1016/0045-6039(81)90019-1.

Abstract

DNA synthesis and DNA polymerase activities were followed in FL cell cultures (clone 5.86) at different stages of differentiation. A temporary block of growth and DNA synthesis was observed after the addition of the inducers (DMSO or HMBA). This delay in the growth and in the DNA synthesis initiation is not observed in cultures of DMSO-resistant variants after treatment with DMSO. In both uninduced and induced cultures, during growth, the DNA gamma-polymerase activity is constant and the alpha-polymerase activity is strictly dependent on the DNA synthesis rate. On the other hand, a different behaviour between induced and uninduced cultures is observed for the DNA polymerase beta: its activity is constant in uninduced cultures, whereas it changes in induced cultures at various stages of differentiation, dropping to lower values at early times and rising to values similar to those observed in uninduced cultures at later times. This behaviour is not observed in cultures of a DMSO-resistant variant clone: in this case the beta-polymerase activity is constant in both the absence and the presence of DMSO or HMBA.

摘要

在FL细胞培养物（克隆5.86）分化的不同阶段，对DNA合成和DNA聚合酶活性进行了跟踪研究。添加诱导剂（二甲基亚砜或六亚甲基双乙酰胺）后，观察到生长和DNA合成出现暂时阻滞。在用二甲基亚砜处理后，在二甲基亚砜抗性变体的培养物中未观察到这种生长和DNA合成起始的延迟。在未诱导和诱导的培养物中，生长期间DNAγ-聚合酶活性恒定，而α-聚合酶活性严格依赖于DNA合成速率。另一方面，对于DNA聚合酶β，在诱导和未诱导的培养物中观察到不同的行为：其活性在未诱导的培养物中恒定，而在诱导的培养物中，在分化的各个阶段发生变化，在早期降至较低值，在后期升至与未诱导培养物中观察到的值相似的值。在二甲基亚砜抗性变体克隆的培养物中未观察到这种行为：在这种情况下，无论有无二甲基亚砜或六亚甲基双乙酰胺，β-聚合酶活性均恒定。