Billingsley G D, Cox D W
Am Rev Respir Dis. 1980 Jan;121(1):161-4. doi: 10.1164/arrd.1980.121.1.161.
A decreased concentration of alpha 1-antitrypsin is associated with a high risk for obstructive lung disease. We measured the elastase inhibitory capacity, the most important biologic measure of alpha 1-AT function, using a natural substrate. The gel plate assay that we developed uses a commercial gelatin film and is more sensitive, faster, and cheaper than similar elastin-elastase methods. In serum samples from 76 patients with emphysema, there was a high correlation between the immunologically measured alpha 1-AT and the elastase inhibitory capacity. There was no evidence for a functionally deficient alpha 1-AT in any of these patients.
α1-抗胰蛋白酶浓度降低与阻塞性肺病的高风险相关。我们使用天然底物测量了弹性蛋白酶抑制能力，这是α1-抗胰蛋白酶功能的最重要生物学指标。我们开发的凝胶平板测定法使用商业明胶膜，比类似的弹性蛋白-弹性蛋白酶方法更灵敏、更快且更便宜。在76例肺气肿患者的血清样本中，免疫测定的α1-抗胰蛋白酶与弹性蛋白酶抑制能力之间存在高度相关性。在这些患者中没有证据表明存在功能缺陷的α1-抗胰蛋白酶。
