Ledney G D, Stewart D A, Exum E D
J Trauma. 1980 Feb;20(2):141-7. doi: 10.1097/00005373-198002000-00007.
The hypothesis that wound trauma produces either selective or total organ cell population alterations in the quantities of lympho-myeloproliferative elements in the major hematocytopoietic centers was tested. Mice were subjected to a 4% body surface skin wound on the anterior dorsum; their tissues were assayed 24 hours later for their total cellularity and proliferative potential. In wounded mice, the marrow cellularity was significantly decreased but this did not result in lower stem cell (CFU-s) and progenitor cell (CFU-c and M-CFC) quantities. However, there were significant selective and total population reductions in cells responsive to T and B cell mitogens. Splenic cellularity and stem cell quantities in wounded mice did not differ from control-treated animals. However, there were both selective and total population reductions in splenic CFU-c and M-CFC. Wounding resulted in a greater splenic T cell response to phytohemagglutinin PHA, while the responses to concanavalin-A (CON-A) were similar to controls. The splenic B lymphocyte population was specifically increased. Wounding resulted in a significant thymic hypocellularity. This was mirrored by a selective decrease in M-CFC and a total cell population reduction in response to T cell mitogens.
对伤口创伤是否会在主要造血中心引起淋巴细胞 - 骨髓增殖元件数量的选择性或全面器官细胞群体改变这一假说进行了测试。给小鼠背部前方造成4%体表面积的皮肤伤口；24小时后检测其组织的总细胞数和增殖潜力。在受伤小鼠中，骨髓细胞数显著减少，但这并未导致干细胞（CFU - s）和祖细胞（CFU - c和M - CFC）数量降低。然而，对T细胞和B细胞有丝分裂原反应的细胞存在显著的选择性和总体数量减少。受伤小鼠的脾脏细胞数和干细胞数量与对照处理的动物无差异。然而，脾脏CFU - c和M - CFC存在选择性和总体数量减少。创伤导致脾脏T细胞对植物血凝素PHA的反应增强，而对刀豆蛋白A（CON - A）的反应与对照相似。脾脏B淋巴细胞群体特异性增加。创伤导致胸腺细胞数显著减少。这反映在M - CFC的选择性减少以及对T细胞有丝分裂原反应的总细胞群体减少上。
