Taussig M J, Holliman A, Wright L J
Immunology. 1980 Jan;39(1):57-60.
The AKR thymoma line BW 5147 has been successfully hybridized with the IgM-bearing (BALB/c x NZB)F1 B lymphoma line WEHI 231. In the hybrids formed, T-cell characteristics were dominant, i.e. there was no expression of IgM but continued expression of Thy-1.1 in eight out of eight lines. Moreover, in two out of eight lines, the Thy-1.2 allele was also expressed. We conclude that, in its ability to hybridize, BW 5147 is not restricted to cells of similar ontogenetic origin (i.e. T cells) and that fusion with the thymoma can lead to suppression of B-cell gene expression and derepression of genes for T-cell markers.
AKR胸腺瘤细胞系BW 5147已成功与携带IgM的(BALB/c×NZB)F1 B淋巴瘤细胞系WEHI 231杂交。在形成的杂交细胞中,T细胞特征占主导,即八株杂交细胞系中均无IgM表达,但Thy-1.1持续表达。此外,八株杂交细胞系中有两株还表达了Thy-1.2等位基因。我们得出结论,就杂交能力而言,BW 5147并不局限于具有相似个体发生起源的细胞(即T细胞),并且与胸腺瘤的融合可导致B细胞基因表达受抑制以及T细胞标志物基因的去抑制。