Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction.

We have established 16 independent hybrid lines derived from a fusion between an AKR thymoma and mixed leukocyte reaction-stimulated C57BL/6 spleen cells. The hybrids express both parental H-2 alleles and show hybrid glucose-6-phosphate isomerase activity. Two of the lines showed Fc receptor activity not expressed in the parental thymoma. The AKR Thy-1.1 allele was expressed in all hybrid lines, but no Thy-1.2 could be detected. No killing activity was retained in the hybrid cells, probably because Sendai virus-mediated fusion of killer cells resulted in the lysis of the thymoma fusion partner.