Köhler G, Lefkovits I, Elliott B, Coutinho A
Eur J Immunol. 1977 Nov;7(11):758-61. doi: 10.1002/eji.1830071103.
We have established 16 independent hybrid lines derived from a fusion between an AKR thymoma and mixed leukocyte reaction-stimulated C57BL/6 spleen cells. The hybrids express both parental H-2 alleles and show hybrid glucose-6-phosphate isomerase activity. Two of the lines showed Fc receptor activity not expressed in the parental thymoma. The AKR Thy-1.1 allele was expressed in all hybrid lines, but no Thy-1.2 could be detected. No killing activity was retained in the hybrid cells, probably because Sendai virus-mediated fusion of killer cells resulted in the lysis of the thymoma fusion partner.
我们建立了16个独立的杂交细胞系，这些细胞系源自AKR胸腺瘤与混合淋巴细胞反应刺激的C57BL/6脾细胞之间的融合。这些杂交细胞系表达双亲的H-2等位基因，并表现出杂交型葡萄糖-6-磷酸异构酶活性。其中两个细胞系表现出亲代胸腺瘤中未表达的Fc受体活性。所有杂交细胞系均表达AKR Thy-1.1等位基因，但未检测到Thy-1.2。杂交细胞中未保留杀伤活性，这可能是因为仙台病毒介导的杀伤细胞融合导致胸腺瘤融合伙伴细胞裂解。
