Shaham M, Becker Y, Cohen M M
Cytogenet Cell Genet. 1980;27(2-3):155-61. doi: 10.1159/000131476.
Cocultivation of plasma and lymphocytes from ataxia telangiectasia (AT) patients with those of normal individuals resulted in a significant increase in chromosomal damage in the normal cells. Tissue culture medium used to cultivate AT skin fibroblasts also significantly increased chromosome breakage in PHA-stimulated normal lymphocytes. This clastogenic effect was maximal when the "conditioned medium" was 8-9 days old. A similar effect was not observed with medium derived from normal skin fibroblasts. These observations suggest the presence of a clastogenic factor in the plasma of AT patients which may also be produced by AT skin fibroblasts in culture.
将共济失调毛细血管扩张症（AT）患者的血浆和淋巴细胞与正常个体的血浆和淋巴细胞共同培养，导致正常细胞中的染色体损伤显著增加。用于培养AT皮肤成纤维细胞的组织培养基也显著增加了PHA刺激的正常淋巴细胞中的染色体断裂。当“条件培养基”培养8 - 9天时，这种致断裂效应最大。从正常皮肤成纤维细胞获得的培养基未观察到类似效应。这些观察结果表明，AT患者血浆中存在一种致断裂因子，培养中的AT皮肤成纤维细胞也可能产生这种因子。
