Azar H A, Hansen C T, Costa J
J Natl Cancer Inst. 1980 Aug;65(2):421-30.
A new "nude" mouse model was developed by successive crossings and backcrossings between athymic nu/nu mice, on an N:NIH(S) background, and female CBA/N mice that have an X-linked immune defect in B-lymphocyte function. The resulting doubly congenic N:NIH(S(II-nu/nu mice maintained the marked thymic hypoplasia and poor development of hair of the nu/nu mice. In contrast to nu/nu and CBA/N mice, in this new mouse model both T-cell zones of lymph nodes and the spleen were depleted of lymphocytes. Lymphocytic follicles were rare and diminutive; not germinal centers were noted. The nodal cortical and paracortical areas were represented principally by connective tissue, endothelial cells, and macrophages, including giant multinucleated cells. No medullary cords were recognized. The mice with combined immunodeficiency supported the growth of human tumor xenografts and were susceptible to murine viral hepatitis.
一种新的“裸”鼠模型是通过在N:NIH(S)背景的无胸腺nu/nu小鼠与具有B淋巴细胞功能X连锁免疫缺陷的雌性CBA/N小鼠之间进行连续杂交和回交而培育出来的。由此产生的双重同源N:NIH(S(II-nu/nu小鼠保留了nu/nu小鼠明显的胸腺发育不全和毛发发育不良的特征。与nu/nu和CBA/N小鼠不同的是，在这种新的小鼠模型中，淋巴结和脾脏的T细胞区均无淋巴细胞。淋巴细胞滤泡稀少且微小；未观察到生发中心。淋巴结皮质和副皮质区域主要由结缔组织、内皮细胞和巨噬细胞组成，包括巨大的多核细胞。未识别出髓索。具有联合免疫缺陷的小鼠支持人肿瘤异种移植物的生长，并且易患鼠病毒性肝炎。
