Raichvarg D, Hatat D, Agneray J
Biomedicine. 1980 Apr;33(2):52-5.
A scale of response, called phagocytic index (PI), is established to study the phagocytic function by measure of the Nitrobluetetrazoluim (NBT) reduction. 0 % and 100 % opsonic activity must be evaluated before the determination of PI which is calculated as follows: (Formula: see text) OD 0% vary with leukocyte species and the NBT batches, OD 100 % is depended on bacteria strains and nature of cells. Therefore PI must be performed in a system strictly characterized for bacteria, cells, and batch of NBT used. PI provides a better evaluation of bacterial phagocytosis as shown with antisera from rabbits immunized with Haemophilus influenzae.
建立了一种称为吞噬指数（PI）的反应量表，通过测量硝基蓝四氮唑（NBT）还原情况来研究吞噬功能。在测定PI之前，必须评估0%和100%的调理活性，PI的计算方法如下：（公式：见正文）OD 0%随白细胞种类和NBT批次而变化，OD 100%取决于细菌菌株和细胞性质。因此，PI必须在一个针对所用细菌、细胞和NBT批次进行严格表征的系统中进行。如用流感嗜血杆菌免疫的兔抗血清所示，PI能更好地评估细菌吞噬作用。
