Raichvarg D, Brossard C, Agneray J
Pathol Biol (Paris). 1980 May;28(5):299-303.
Opsonins may protect against infections. In this study, the different parameters of opsonization assay are strictly standardized. Results are expressed as percentage of opsonization. 0% and 100% opsonization must be established. The experiment without immunserum is used as the 0% opsonization. To determine the 100% opsonization an excess of reducing capacity is required. This is provided by crushed leukocytes. Rabbits are immunised by bacterial extract from Haemophilus influenzae type a. The hemagglutinins are detected in sera earlier (D + 30) and longer than the opsonic activity. Phagocytosis increases from the second month following the immunization. Percentage of opsonization is maintained at about 50% up to the sixth month. Alternatively, the percentage of phagocytosis is decreasing when heterologous bacteria are used. The serum thermolabile factors are necessary for the response. The phagocytic capacity is not modified whether leukocytes are obtained from immunized rabbits or not.
调理素可能有助于预防感染。在本研究中，调理作用测定的不同参数被严格标准化。结果以调理作用的百分比表示。必须确定0%和100%的调理作用。无免疫血清的实验用作0%的调理作用。为了确定100%的调理作用，需要过量的还原能力。这由破碎的白细胞提供。用a型流感嗜血杆菌的细菌提取物免疫兔子。血凝素在血清中比调理活性更早（D + 30）且更长时间被检测到。吞噬作用从免疫后的第二个月开始增加。调理作用的百分比在第六个月前维持在约50%。或者，当使用异源细菌时，吞噬作用的百分比会下降。血清热不稳定因子是该反应所必需的。无论白细胞是否取自免疫兔子，吞噬能力均未改变。
