Polin R A, Kennett R
In Vitro. 1980 Jul;16(7):575-80. doi: 10.1007/BF02618381.
Pokeweed mitogen (PWM) and ricin are both lectins derived from plant seeds. They are glycoproteins and share the ability to agglutinate a variety of animal cells including erythrocytes. The effect of these two lectins on protein synthesis was studied in four long-term lymphoblastoid lines (8866 and GM1531, which are B cell lines; and CCRF/CEM and MOLT 4, which are T-cell lines). Ricin (50 micrograms/ml) completely inhibited protein synthesis by 2 hr in both B-cell and T-cell lines as measured by the uptake to [3H]leucine. The PWM appeared more specific and at a concentration of 500 micrograms/ml inhibited protein synthesis only in B-cell lines (8866 and GM1531). This effect was maximal at 5 hr. To investigate the reason for the differential effect of PWM on T and B cells, 125I-labeled PWM was incubated with 8866, MOLT 4, and CCRF/CEM to see if a significant difference in binding to B cells and T cells could be demonstrated. It does not appear that differential effect on T and B cells is due to a difference in the amount of PWM bound. On the other hand it is possible that the B cells may bind some toxic subcomponent of the PWM preparation that the T cells do no bind because of a difference in composition or arrangement of cell surface glycoproteins.
商陆有丝分裂原（PWM）和蓖麻毒素都是从植物种子中提取的凝集素。它们是糖蛋白，都具有凝集包括红细胞在内的多种动物细胞的能力。在四个长期淋巴细胞系（8866和GM1531，为B细胞系；CCRF/CEM和MOLT 4，为T细胞系）中研究了这两种凝集素对蛋白质合成的影响。蓖麻毒素（50微克/毫升）在2小时内完全抑制了B细胞系和T细胞系中的蛋白质合成，这通过[3H]亮氨酸的摄取来测定。PWM表现出更强的特异性，在浓度为500微克/毫升时仅抑制B细胞系（8866和GM1531）中的蛋白质合成。这种作用在5小时时达到最大。为了研究PWM对T细胞和B细胞产生不同作用的原因，将125I标记的PWM与8866、MOLT 4和CCRF/CEM一起孵育，以观察在与B细胞和T细胞的结合上是否能显示出显著差异。对T细胞和B细胞的不同作用似乎不是由于结合的PWM量的差异。另一方面，有可能B细胞可能结合了PWM制剂中的一些有毒亚成分，而T细胞由于细胞表面糖蛋白的组成或排列不同而不结合。
