Pimsler M, Forman J
Immunogenetics. 1980;11(2):111-21. doi: 10.1007/BF01567777.
Lymph-node cells from H-2 allogeneic, intra-H-2 recombinant and H-2 mutant congenic strains were sensitized in limiting dilution cultures to quantitate the cytotoxic T-lymphocyte precursor frequencies (CTL.Pf) against antigens encoded by different regions of the H-2 complex. When four H-2Kb mutants of C57BL/6 (B6) were tested, we observed anti-B6 CTL.Pf that were as high or higher than those of recombinant strains which differ from B6 at the K end of the H-2 complex. Relative to strains completely H-2 allogeneic to B6, the CTL.Pf in H-2bm3 and H-2bm5 averaged 40--50 percent, and H-2bm8 averaged 140 percent. Recombinant strains B10.A (4R) and B10.D2 (R103), which differ from B6 at the K end of the H-2 complex, averaged 60 percent of the completely H-2 allogeneic value. Since the mutant and wild-type gene products have no serological and minimal structural differences relative to other alleles at H-2K, these results indicate that the CTL.Pf does not increase with increasing H-2 antigenic disparity between andy two strains. Rather, the data suggests that the T-cell receptor repertoire recognizes those H-2 molecules or determinants closest to self.
对来自H-2同种异体、H-2复合体内重组体和H-2突变同源系的淋巴结细胞在有限稀释培养中进行致敏，以定量针对由H-2复合体不同区域编码的抗原的细胞毒性T淋巴细胞前体频率（CTL.Pf）。当检测C57BL/6（B6）的四个H-2Kb突变体时，我们观察到抗B6 CTL.Pf与在H-2复合体K端与B6不同的重组系一样高或更高。相对于与B6完全H-2异体的品系，H-2bm3和H-2bm5中的CTL.Pf平均为40%-50%，H-2bm8平均为140%。在H-2复合体K端与B6不同的重组系B10.A（4R）和B10.D2（R103），平均为完全H-2异体值的60%。由于突变型和野生型基因产物与H-2K处的其他等位基因相比没有血清学差异且结构差异最小，这些结果表明CTL.Pf不会随着任何两个品系之间H-2抗原差异的增加而增加。相反，数据表明T细胞受体库识别那些最接近自身的H-2分子或决定簇。
