Eggers A E, Hibbard C A, Civin C I, Wunderlich J R
J Immunol. 1980 Oct;125(4):1737-44.
C57BL/6 spleen cells immunized in vitro against syngeneic methylcholanthrene-induced sarcoma cells(MC-1 cells) modified with various chemical reagents show cytotoxic activity against unmodified MC-1 cells in a short-term 51Cr release assay, whereas unmodified MC-1 cells are nonimmunogenic. The effector cells cross-react widely with many other fibroblastic and epithelioid tumors, even those that are not H-2 matched, as well as with some nonneoplastic cells. Priming mice in vivo with a hapten leads to enhanced anti-tumor cytotoxicity developed by spleen cells from these mice immunized in vitro against tumor cells modified with the same hapten. Cytotoxic activity is largely, but not completely, removed by treatment with anti-theta serum and C. The existence of suppressor cells that can inhibit an anti-tumor response is demonstrasted in in vitro immunizations of mixtures of spleen cells from normal mice and mice primed with mitomyhcin-treated tumor cells, the latter suppressing the former.
在体外针对用各种化学试剂修饰的同基因甲基胆蒽诱导的肉瘤细胞（MC-1细胞）免疫的C57BL/6脾细胞，在短期51Cr释放试验中显示出对未修饰的MC-1细胞的细胞毒性活性，而未修饰的MC-1细胞是非免疫原性的。效应细胞与许多其他成纤维细胞样和上皮样肿瘤广泛交叉反应，甚至包括那些H-2不匹配的肿瘤，以及一些非肿瘤细胞。用半抗原在体内致敏小鼠会导致这些小鼠的脾细胞在体外针对用相同半抗原修饰的肿瘤细胞免疫后产生增强的抗肿瘤细胞毒性。细胞毒性活性在很大程度上但并非完全被抗θ血清和补体处理所消除。在正常小鼠和用丝裂霉素处理的肿瘤细胞致敏的小鼠的脾细胞混合物的体外免疫中，证明了存在可抑制抗肿瘤反应的抑制细胞，后者抑制前者。
