Leav I, Merk F B, Ofner P, Goodrich G, Kwan P W, Stein B M, Sar M, Stumpf W E
Am J Pathol. 1978 Oct;93(1):69-92.
Prostatic response to administered estradiol-17beta 17-cyclopentylpropionate (ECP) was studied in castrated or hypophysectomized dogs. Ultrastructural investigation identified two types of epithelial cells, squamous and modified glandular, that resulted from estrogen action. Squamous cells, which predominated, appeared to evolve from basal reserve cells. Estrogen-modified glandular cells have features attributable to estrogen and previous androgen stimulation. Thaw-mount autoradiography of explants located radiolabeled estrogen in prostatic epithelium and stroma of normal and ECP-treated animals, suggesting a role of estrogen in prostatic homeostasis. Epithelium from ECP-treated dogs incorporated 3H-thymidine, indicating that estrogen promotes DNA synthesis in these cells. Greatly enhanced conversion of radiotestosterone to 4-androstene-3,17-dione, with sharply decreased formation of 5alpha-reduced hydroxylation products, and attendant elevation in estradiol-17beta oxidoreductase activity are metabolic markers attributable to the induced squamous and responding stromal cells. The duality of prostatic response to sex hormones is apparent from observations that estrogen stimulates regressed glandular epithelium which had undergone androgen-mediated differentiation before ablation.
在去势或垂体切除的犬中研究了前列腺对所给予的17β-雌二醇17-环戊丙酸酯（ECP）的反应。超微结构研究确定了两种类型的上皮细胞，即鳞状细胞和改良腺细胞，它们是雌激素作用的结果。占主导地位的鳞状细胞似乎由基底储备细胞演变而来。雌激素修饰的腺细胞具有可归因于雌激素和先前雄激素刺激的特征。对正常和ECP处理动物的前列腺上皮和基质进行解冻装片放射自显影，发现放射性标记的雌激素，这表明雌激素在前列腺内环境稳定中起作用。ECP处理犬的上皮细胞掺入3H-胸腺嘧啶，表明雌激素促进这些细胞中的DNA合成。放射性睾酮向4-雄烯-3,17-二酮的转化大大增强，5α-还原羟基化产物的形成急剧减少，同时雌二醇-17β氧化还原酶活性升高，这些是可归因于诱导的鳞状细胞和反应性基质细胞的代谢标志物。从雌激素刺激在切除前经历雄激素介导分化的退化腺上皮这一观察结果可以明显看出前列腺对性激素反应的双重性。
