Boss M A, Delia D, Robinson J B, Greaves M F
Blood. 1980 Nov;56(5):910-6.
The cell surface antigenic phenotype of HL-60, a human acute promyelocytic leukemia cell line, has been analyzed before and after maturation induction with dimethylsulfoxide (DMSO) using a panel of markers including a "library" of monoclonal antibodies and "conventional" antisera in conjunction with the fluorescene-activated cell sorter. HL-60 cells express granulocyte and "leukocyte" differentiation antigens but not antigens of the lymphoid, platelet, and erythroid lineages. DMSO-induced morphological maturation was found to be associated with a decrease in the proportion of cells in mitotic cycle, induction of C3d receptors, increased expression of granulocytic and leukocyte antigens, and diminished expression of HLA-A,B,C and beta 2-microglobulin determinants. HL-60 cells have no detectable expression of HLA-DR-associated determinants as assayed by rabbit anti-p28,33 monoclonal anti-HLA-DR (monomorphic determinant), and HLA-DRw typing alloantisera. The relationship of these changes in cell surface properties to normal granulocytic differentiation is discussed.
利用一组标志物，包括一个单克隆抗体“文库”和“传统”抗血清，并结合荧光激活细胞分选仪，对人急性早幼粒细胞白血病细胞系HL - 60在二甲基亚砜（DMSO）诱导成熟前后的细胞表面抗原表型进行了分析。HL - 60细胞表达粒细胞和“白细胞”分化抗原，但不表达淋巴系、血小板系和红系的抗原。发现DMSO诱导的形态成熟与有丝分裂周期中细胞比例的降低、C3d受体的诱导、粒细胞和白细胞抗原表达的增加以及HLA - A、B、C和β2 - 微球蛋白决定簇表达的减少有关。用兔抗p28,33单克隆抗HLA - DR（单态决定簇）和HLA - DRw分型同种抗血清检测发现，HL - 60细胞没有可检测到的HLA - DR相关决定簇的表达。本文讨论了细胞表面特性的这些变化与正常粒细胞分化的关系。
