Wright D A, Richards C M
Genetics. 1983 Feb;103(2):249-61. doi: 10.1093/genetics/103.2.249.
Crosses involving one heterozygous parent were performed to test the inheritance of enzymes in the leopard frog, Rana pipiens. After metamorphosis, offspring were sexed and tissue extracts from them were analyzed by gel electrophoresis. Enzyme genotype and sex showed independent assortment for 10 of 12 enzymes heterozygous in the male parent. However, among the offspring of males heterozygous for peptidase C (Pep-C) or superoxide dismutase 1 (SOD-1), male progeny tend to inherit one allele, whereas female progeny tend to inherit the other. Data from several different crosses yield recombination frequencies of 8.6% between sex and SOD-1, 6.9% between SOD-1 and Pep-C and 12.1% between sex and Pep-C. When the female parent is heterozygous for these enzymes no significant difference is seen, in the offspring, between male and female homozygotes and heterozygotes. These results confirm that males are the heterogametic sex in R. pipiens and suggest that sex is determined by a small number of genes on otherwise identical X and Y chromosomes.
进行涉及一个杂合亲本的杂交实验，以测试豹蛙（北美林蛙）中酶的遗传情况。变态发育后，对后代进行性别鉴定，并通过凝胶电泳分析它们的组织提取物。对于雄性亲本中杂合子的12种酶中的10种，酶基因型和性别表现出独立分配。然而，在肽酶C（Pep-C）或超氧化物歧化酶1（SOD-1）杂合的雄性后代中，雄性子代倾向于继承一个等位基因，而雌性子代倾向于继承另一个等位基因。来自几个不同杂交组合的数据显示，性别与SOD-1之间的重组频率为8.6%；SOD-1与Pep-C之间为6.9%；性别与Pep-C之间为12.1%。当雌性亲本对这些酶为杂合子时，在后代中，雄性和雌性纯合子与杂合子之间没有显著差异。这些结果证实了雄性是北美林蛙中的异配性别，并表明性别由其他方面相同的X和Y染色体上的少数基因决定。
