Eandi M, Ricci Gamalero S
Boll Soc Ital Biol Sper. 1980 Jan 15;56(1):1-7.
The effect "in vitro" and "in vivo" of local anesthetics is normally evaluated at fixed time or considering the time required to obtain a per cent effect. We have attempted to estimate the dose/effect correlation at steady state using "in vitro" preparation of sciatic nerve-trunk of frog. Such an experiment is possible only if the concentrations of drugs tested are lower than those used in previous methods, so as not to inhibit the nerve fibres completely. The drugs tested were: lidocaine, procaine, promethazine and imipramine. Using linear regression analysis, it is possible to compare both the DE50 of the drugs tested and slopes of linear regression. In this manner we can better characterize the pharmacodynamic properties of local anesthetics.
局部麻醉药的“体外”和“体内”效应通常在固定时间进行评估，或者考虑获得一定百分比效应所需的时间。我们试图利用青蛙坐骨神经干的“体外”制剂来估计稳态下的剂量/效应相关性。只有当所测试药物的浓度低于先前方法中使用的浓度时，这样的实验才有可能进行，以免完全抑制神经纤维。所测试的药物有：利多卡因、普鲁卡因、异丙嗪和丙咪嗪。使用线性回归分析，可以比较所测试药物的半数有效剂量（DE50）和线性回归的斜率。通过这种方式，我们可以更好地表征局部麻醉药的药效学特性。
