兔抗大鼠、兔抗犬和兔抗人淋巴细胞血清所识别抗原的组织分布定量研究。
Quantitative studies of the tissue distribution of the antigens recognized by rabbit anti-rat, -dog and -human lymphocyte sera.
作者信息
Dalchau R, Fabre J W
出版信息
Clin Exp Immunol. 1980 Jul;41(1):182-7.
Rabbit anti-rat, -dog and -human ALS was analysed using quantitative absorptions and indirect 125I anti-immunoglobulin-binding assays to determine the amount of antibody directed against different types of antigen. The aim was (a) to determine what proportion of the antibodies was directed against leucocyte-specific antigens and (b) to see if the type of leucocyte-specific antigen recognized varied either with the species or the cell type used as the immunogen. The results showed that anti-thymocyte sera in all three species showed the same high degree of leucocyte specificity. Marked differences in the type of leucocyte-specific antigen recognized were found when anti-thymocyte and anti-lymph node lymphocyte sera were compared. A comparison of the leucocyte-specific antigens recognized by anti-dog and -human thymocyte sera showed quantitative but not qualitative differences. The relevance of these results to the current failure of ALS in clinical practice and to the problems of ALS standardization are discussed.
使用定量吸收法和间接¹²⁵I抗免疫球蛋白结合测定法分析兔抗大鼠、抗犬和抗人抗淋巴细胞血清（ALS），以确定针对不同类型抗原的抗体量。目的是：（a）确定抗体中针对白细胞特异性抗原的比例；（b）观察所识别的白细胞特异性抗原类型是否因用作免疫原的物种或细胞类型而异。结果表明，所有三个物种的抗胸腺细胞血清均表现出相同高度的白细胞特异性。当比较抗胸腺细胞血清和抗淋巴结淋巴细胞血清时，发现所识别的白细胞特异性抗原类型存在显著差异。比较抗犬和抗人胸腺细胞血清所识别的白细胞特异性抗原，发现存在定量而非定性差异。讨论了这些结果与目前ALS在临床实践中的失败以及ALS标准化问题的相关性。
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