肉芽组织提取物对胶原蛋白合成的影响。

Effects of granulation-tissue extracts on collagen synthesis.

作者信息

Kulonen E, Potila M

出版信息

Exp Pathol (Jena). 1980;18(7-8):456-61. doi: 10.1016/s0014-4908(80)80047-0.

Abstract

The effect of granulation-tissue extract on the synthesis of collagen in embryonic-chick tendon cells was found to depend on (i) the developmental phase of the granulation tissue, (ii) the concentration of the extract and (iii) the presence of diffusible components in it. The suppressing effect was maximal with concentrated extracts from 2--3-week granulation tissue (where the collagen synthesis is also maximal). The suppressing factor could be enriched by gel filtration chromatography. Cultured human synovial cells were affected like the embryonic-chick cells. When the sponge implant was imbibed with granulation-tissue extract in advance, the developing granuloma contained less collagen than the control. The stimulating effects with dilute extracts from granulation tissue were due to diffusible components. Blood serum and rheumatoid synovial fluid samples also stimulated collagen synthesis in granulation-tissue slices, less after dialysis.

摘要

发现肉芽组织提取物对胚胎鸡肌腱细胞中胶原蛋白合成的影响取决于

（i）肉芽组织的发育阶段；（ii）提取物的浓度；（iii）其中可扩散成分的存在。来自2 - 3周肉芽组织的浓缩提取物（此时胶原蛋白合成也最大）的抑制作用最大。抑制因子可通过凝胶过滤色谱法富集。培养的人滑膜细胞与胚胎鸡细胞受到的影响相似。当海绵植入物预先用肉芽组织提取物浸润时，形成的肉芽肿中的胶原蛋白含量比对照少。肉芽组织稀释提取物的刺激作用归因于可扩散成分。血清和类风湿性滑液样本也刺激肉芽组织切片中的胶原蛋白合成，透析后刺激作用减弱。