Usinger W R, Splitter G A
Immunol Commun. 1980;9(7):693-703. doi: 10.3109/08820138009066018.
The treatment of bovine peripheral blood mononuclear cells with rabbit anti-bovine immunoglobulin, goat anti-rabbit immunoglobulin (GAR) and complement resulted in the specific lysis of all surface immunoglobulin (SIg) bearing B lymphocytes. No SIg+ lymphocytes were detected after 12 hours of culture following lytic treatment, whereas cells treated with antibody without complement readily stained for SIg. The percentage of cells forming E-rosettes or binding peanut agglutinin (PNA) increased following lysis. The percentage of latex-ingesting monocytes also increased after lysis even though many of these cells had cytophilic Ig. B lymphocyte-depleted (T cell-enriched) populations cultured with phytohemagglutinin (PHA) and concanavalin A (Con A) were never less reactive than unseparated cells. No differences in background mitosis was observed for these 2 cells preparations. These results suggest that bovine SIg- cells do not require B lymphocytes to respond to PHA and Con A and that lysis of B lymphocytes does not alter the responsiveness of the recovered SIg- cells.
用兔抗牛免疫球蛋白、山羊抗兔免疫球蛋白（GAR）和补体处理牛外周血单核细胞，导致所有带有表面免疫球蛋白（SIg）的B淋巴细胞发生特异性裂解。裂解处理后培养12小时未检测到SIg +淋巴细胞，而用无补体抗体处理的细胞很容易被SIg染色。裂解后形成E花环或结合花生凝集素（PNA）的细胞百分比增加。即使许多这些细胞具有嗜细胞性Ig，摄取乳胶的单核细胞百分比在裂解后也增加。用植物血凝素（PHA）和刀豆球蛋白A（Con A）培养的B淋巴细胞耗竭（T细胞富集）群体的反应性从未低于未分离的细胞。这两种细胞制剂在背景有丝分裂方面未观察到差异。这些结果表明，牛SIg -细胞对PHA和Con A的反应不需要B淋巴细胞，并且B淋巴细胞的裂解不会改变回收的SIg -细胞的反应性。
