Reimann J, Diamantstein T
Immunobiology. 1980 Dec;157(4-5):437-49. doi: 10.1016/S0171-2985(80)80013-1.
Polyclonally activated splenic lymphocytes (generated in mitogen-stimulated cultures) were transferred to syngeneic or autologous recipient mice. Injection of cells into the footpad of syngeneic recipients induced a regional response in the ipsilateral popliteal lymph node; intravenous cell transfer elicited a systemic splenomegaly reaction. These reactions displayed a linear log number of transferred cells/response relationship in syngeneic and autologous systems. The kinetic and magnitude of the regional response to syngeneic lymphoblasts ands to allogeneic spleen cells were comparable. No difference was apparent in the phenomenology of the in-vivo responses to syngeneic lymphoblasts induced by various T- or B-cell mitogens. The in-vivo response was: 1. stimulated by large-size lymphoblasts; and 2. mediated by host T cells. Data excluded a direct involvement of mitogen or heterologous serum constituents in tissue culture medium in the described reaction. Experimental evidence argues against the involvement of virus components in the observed phenomenon.
多克隆激活的脾淋巴细胞（在有丝分裂原刺激的培养物中产生）被转移到同基因或自体受体小鼠体内。将细胞注射到同基因受体的足垫中会在同侧腘窝淋巴结诱导局部反应；静脉内细胞转移引发全身性脾肿大反应。在同基因和自体系统中，这些反应显示出转移细胞数量/反应的线性对数关系。对同基因淋巴母细胞和同种异体脾细胞的局部反应的动力学和幅度相当。由各种T细胞或B细胞有丝分裂原诱导的对同基因淋巴母细胞的体内反应的现象学没有明显差异。体内反应为：1. 由大尺寸淋巴母细胞刺激；2. 由宿主T细胞介导。数据排除了组织培养基中的有丝分裂原或异源血清成分直接参与所述反应。实验证据反对病毒成分参与观察到的现象。
