Reyes F, Lahoz R, Vazquez C
Can J Microbiol. 1980 Sep;26(9):1120-5. doi: 10.1139/m80-185.
In the autolytic phase of growth Schizophyllum commune lost 62% of its dry weight in 70 days of incubation. The variations in the activity of some lytic enzymes were studied in the culture fluid and mycelial extracts during growth and autolysis of this fungus. The enzymes 1,3-beta-glucanase (exoglucanase), 1,3(4)-beta-glucanase (endoglucanase), alpha-amylase, and invertase behaved in the same way in culture fluid and mycelial extract, but their activities were much higher in the culture fluid. The enzyme activities increased during autolysis, but then decreased at the end of this period except in the case of alpha-amylase which remained high. It was only possible to detect 1,6-beta-glucanase, cellulase, and polygalacturonase activities at certain times during the autolytic phase of growth. The enzyme chitinase was not detected and 1,3-alpha-glucanase (S-glucanase) occurred in the mycelial extract at a higher concentration than in the culture fluid. A decrease in the activity of this enzyme in the mycelial extract and an increase in the culture fluid occurred during autolysis. The enzyme 1,3-alpha-glucanase exhibited two optima pH, one at 6.0 and the other at 8.0. The Km value for the latter was 0.02 M at pH 5.5 in borate-citrate-phosphate buffer.
在生长的自溶阶段，裂褶菌在70天的培养期内干重损失了62%。研究了该真菌生长和自溶过程中培养液和菌丝体提取物中一些裂解酶活性的变化。1,3-β-葡聚糖酶（外切葡聚糖酶）、1,3(4)-β-葡聚糖酶（内切葡聚糖酶）、α-淀粉酶和转化酶在培养液和菌丝体提取物中的表现相同，但它们在培养液中的活性要高得多。酶活性在自溶过程中增加，但在该阶段结束时下降，除了α-淀粉酶的活性仍然很高。在生长的自溶阶段，仅在某些时候检测到1,6-β-葡聚糖酶、纤维素酶和多聚半乳糖醛酸酶的活性。未检测到几丁质酶，1,3-α-葡聚糖酶（S-葡聚糖酶）在菌丝体提取物中的浓度高于培养液。自溶过程中，该酶在菌丝体提取物中的活性降低，在培养液中的活性增加。1,3-α-葡聚糖酶表现出两个最适pH值，一个在6.0，另一个在8.0。在硼酸盐-柠檬酸盐-磷酸盐缓冲液中，pH 5.5时后者的Km值为0.02 M。
