Rusthoven J J, Phillips R A
Exp Hematol. 1980 May;8(5):578-83.
The cycle specific drug, hydroxyurea (HU), has previously been used to study the proliferative kinetics of various hemopoietic precursor cells such as granulocyte-macrophage precursors (CFUc) and multipotential stem cells (CFUs). We have investigated the kinetics of another hemopoietic precursor, a T-cell colony (CFUT). When mice are exposed to two doses of HU, separated by 7 h, CFUT are reduced to approximately 30% of the control value. Surviving cells in the bone marrow quickly replenish the population and return it to normal values 2 days after HU treatment. When fresh bone marrow cells or bone marrow cells harvested from mice exposed 24 h earlier to HU are treated in vitro with HU, CFUT activity is reduced by approximately 70%. These data indicate that CFUT is a rapidly proliferating population with a large proportion of the cells always in cell cycle.
周期特异性药物羟基脲（HU）此前已被用于研究各种造血前体细胞的增殖动力学，如粒细胞-巨噬细胞前体（CFUc）和多能干细胞（CFUs）。我们研究了另一种造血前体——T细胞集落（CFUT）的动力学。当小鼠接受两剂间隔7小时的HU时，CFUT减少至对照值的约30%。骨髓中的存活细胞迅速补充群体数量，并在HU治疗后2天将其恢复到正常值。当新鲜骨髓细胞或从24小时前接受HU处理的小鼠收获的骨髓细胞在体外经HU处理时，CFUT活性降低约70%。这些数据表明CFUT是一个快速增殖的群体，其中很大一部分细胞始终处于细胞周期中。
