Radzun H J, Parwaresch M R
Exp Hematol. 1980 Jul;8(6):737-41.
Lysosomal acid phosphatase and acid esterase from highly purified, viable human thymocytes, T lymphocytes, B lymphocytes, granulocytes, monocytes, platelets, and erythrocytes were subjected to direct measurement of activity and to isoelectric focusing in polyacrylamide thin-layer slabs. The typical patterns for each cell line were found to be distinctly different from the results recently presented in this journal. The study described here opens a new possibility for the biochemical identification of cells and their functional derivatives and neoplastic variants.
对来自高度纯化的、有活力的人胸腺细胞、T淋巴细胞、B淋巴细胞、粒细胞、单核细胞、血小板和红细胞的溶酶体酸性磷酸酶和酸性酯酶进行了活性的直接测定以及在聚丙烯酰胺薄层板中的等电聚焦。发现每种细胞系的典型模式与本期刊最近发表的结果明显不同。此处描述的研究为细胞及其功能衍生物和肿瘤变体的生化鉴定开辟了新的可能性。