Merluzzi V J, Faanes R B, Choi Y S
Int J Immunopharmacol. 1980;2(4):341-4. doi: 10.1016/0192-0561(80)90034-x.
Spleen cells from mice treated with cyclophosphamide (150 mg/kg) and cultured at suboptimal concentrations do not generate cytotoxic T lymphocytes to allogeneic tumor cells. The reduced response of spleen cells from cyclophosphamide-treated mice is not due to the elimination of cytotoxic T cell precursors because normal responses are obtained by the addition of Lyt-1+2- T helper cells to the culture system. These results indicate that helper cells, required for the development of cytotoxic T cell responses to tumor alloantigens, are eliminated by cyclophosphamide in the absence of evident toxicity to cytotoxic T cell precursors.
用环磷酰胺（150毫克/千克）处理过的小鼠的脾细胞，在次优浓度下培养时，不会产生针对同种异体肿瘤细胞的细胞毒性T淋巴细胞。环磷酰胺处理过的小鼠脾细胞反应性降低，并非由于细胞毒性T细胞前体被清除，因为通过向培养系统中添加Lyt-1+2-T辅助细胞可获得正常反应。这些结果表明，对肿瘤同种异体抗原产生细胞毒性T细胞反应所需的辅助细胞，在对细胞毒性T细胞前体无明显毒性的情况下，被环磷酰胺清除。
