Golightly M G, Zielske J V, Golub S H
J Clin Lab Immunol. 1980 Nov;4(3):181-8.
The culture of human peripheral blood lymphocytes (PBL) in fetal bovine serum (FBS) supplemented media results in the activation of an amplifier activity for mitogen or antigen induced proliferative responses. T cells are required for the generation of amplifier cells. In addition, this amplifier activity was shown to require a cell with Fc receptors for IgG but lacked Fc receptors for IgM. Therefore, it appears that the FBS induced amplifier cells have the same characteristics as the FBS culture induced NK-like cytotoxic and proliferating cells, i.e. these cells are of T cell origin, Fc gamma positive, and Fc mu negative. These FBS-cultured PBL may be a useful model for studying cell interactions and regulatory phenomena in human lymphoid cells.
在补充有胎牛血清（FBS）的培养基中培养人外周血淋巴细胞（PBL），会导致有丝分裂原或抗原诱导的增殖反应的放大活性被激活。T细胞是产生放大细胞所必需的。此外，已证明这种放大活性需要一种具有IgG的Fc受体但缺乏IgM的Fc受体的细胞。因此，似乎FBS诱导的放大细胞与FBS培养诱导的NK样细胞毒性和增殖细胞具有相同的特征，即这些细胞起源于T细胞，Fcγ阳性，Fcμ阴性。这些FBS培养的PBL可能是研究人淋巴细胞中细胞相互作用和调节现象的有用模型。
