Goto Y, Ohki Y, Shimizu J, Shukuya R
Biochim Biophys Acta. 1981 Feb 13;657(2):383-9. doi: 10.1016/0005-2744(81)90324-7.
The mitochondrial and cytosolic phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxy-lyase(transphosphorylating), EC 4.1.1.32) occurring in the bullfrog (Rana catesbeiana) liver were studied. The enzymes in th two intracellular compartments of both tadpole and adult frog liver were immunologically identical. Both radioactively-labelled forms of the mitochondrial and cytosolic phosphoenolpyruvate carboxykinase from bullfrog liver were imported at the same rate into intact mitochondria in vitro. The mitochondrial and cytosolic enzyme activities did not respond to the administration of glucagon, glucocorticoid, quinolinate and D-mannoheptulose which are known as enhancers of phosphoenolpyruvate carboxykinase, but were found to increase during natural metamorphosis. The former activity was markedly increased in the tadpoles treated with 3,5,3'-triiodothyronine. It was supposed that in the bullfrog liver the phosphoenolpyruvate carboxykinase localized in the mitochondria is of central importance in phosphoenolpyruvate synthesis from oxaloacetate.
对牛蛙（牛蛙）肝脏中存在的线粒体和胞质磷酸烯醇式丙酮酸羧激酶（GTP：草酰乙酸羧基裂解酶（转磷酸化），EC 4.1.1.32）进行了研究。蝌蚪和成年蛙肝脏两个细胞内区室中的酶在免疫学上是相同的。牛蛙肝脏线粒体和胞质磷酸烯醇式丙酮酸羧激酶的两种放射性标记形式在体外以相同的速率导入完整的线粒体。线粒体和胞质酶活性对已知为磷酸烯醇式丙酮酸羧激酶增强剂的胰高血糖素、糖皮质激素、喹啉酸和D-甘露庚酮糖的给药没有反应，但发现在自然变态过程中会增加。在用3,5,3'-三碘甲状腺原氨酸处理的蝌蚪中，前者的活性明显增加。据推测，在牛蛙肝脏中，定位于线粒体的磷酸烯醇式丙酮酸羧激酶在由草酰乙酸合成磷酸烯醇式丙酮酸中起着核心作用。
