Ohki Y, Goto Y, Shukuya R
Biochim Biophys Acta. 1981 Oct 13;661(2):230-4. doi: 10.1016/0005-2744(81)90008-5.
The rates of synthesis and degradation of mitochondrial phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32) in the liver of tadpoles in the two developmental stages (stages VIII and XXII), and in those treated with 3,5,3'-triiodothyronine, were studied by immunochemical techniques. The rate of synthesis of the enzyme was found to be accelerated at 9 h and 6 days after triiodothyronine administration and also during natural metamorphic climax. No difference was observed in the degradation rate of the enzyme between the tadpoles in the two stages, VIII and XXII.
运用免疫化学技术研究了处于两个发育阶段（第八阶段和第二十二阶段）的蝌蚪肝脏中线粒体磷酸烯醇式丙酮酸羧激酶（GTP：草酰乙酸羧基裂解酶（转磷酸化），EC 4.1.1.32）的合成和降解速率，以及用3,5,3'-三碘甲状腺原氨酸处理后的蝌蚪肝脏中该酶的合成和降解速率。结果发现，在给予三碘甲状腺原氨酸后9小时和6天，以及在自然变态高峰期，该酶的合成速率加快。在第八阶段和第二十二阶段的蝌蚪之间，未观察到该酶降解速率的差异。
