Teale J M, Lafrenz D, Klinman N R, Strober S
J Immunol. 1981 May;126(5):1952-7.
In order to determine the extent to which B cells exhibit class commitment, the isotype(s) secreted by individual B cells and their progeny was studied in relation to the isotype(s) expressed on the surface of the B cell. Both primary and secondary B cells were separated according to cell surface isotypes and analyzed under optimal stimulation conditions using the T-dependent splenic focus assay. From these studies the following conclusions conclude be made: a) essentially all mature primary B cells appear to express mu on their surface; b) accordingly, the primary B cell population appears to be devoid of B cells expressing solely IgG on their surface; c) the majority of primary B cells in the presence of optimal T cell help have the potential for multiple class production; d) the secretion of IgA coincides with that of IgM and not IgG in its synthesis by immature B cells; e) a substantial proportion of memory B cells bear mu on their surface; f) memory B cells that lack s-mu are unable to yield clones secreting IgM; and g) a substantial proportion of memory B cells have the potential for multiple class production.
为了确定B细胞表现出类别定向的程度，研究了单个B细胞及其后代分泌的同种型与B细胞表面表达的同种型之间的关系。根据细胞表面同种型分离出原代和二代B细胞，并在最佳刺激条件下使用依赖T细胞的脾灶试验进行分析。从这些研究中可以得出以下结论：a）基本上所有成熟的原代B细胞表面似乎都表达μ链；b）因此，原代B细胞群体似乎没有表面仅表达IgG的B细胞；c）在最佳T细胞辅助存在的情况下，大多数原代B细胞具有产生多种类别的潜力；d）在未成熟B细胞合成过程中，IgA的分泌与IgM而非IgG的分泌同时发生；e）相当一部分记忆B细胞表面带有μ链；f）缺乏s-μ链的记忆B细胞无法产生分泌IgM的克隆；g）相当一部分记忆B细胞具有产生多种类别的潜力。
