Barta O, Hubbert N L
Am J Vet Res. 1978 Aug;39(8):1303-8.
Total complement (C) and its components were assayed in the serum of 8 species of domestic animals, using commercially prepared cellular intermediates of sheep erythrocytes and functionally pure guinea pig and human components of the C system. Testing was done according to methods recommended by the producer for testing human C components. The late-acting components (C6 throug C9) and C1 were detected in carnivorous (dog and cat) and omnivorous (swine) animals. Undetectable or low titers of C4, C2, C3, and C5 were present in large herbivorous animals (cattle, horse, sheep, and goat), indicating major differences in comparison with human or guinea pig components of C. Porcine serum contained an inhibiting substance which interfered with testing C2 and later-acting components at serum dilutions up to 1:100. All components except C2 were detected in chicken serum. The binding or activation (or both) of C4, C2, C3, and C5 is more species specific than is the binding or activation (or both) of other components. Requirements for species specificity between antibody and C1 were not detected. Presence of C1 inactivator was detected in bovine, caprine, equine, and ovine sera. The CH50 (50% hemolysis) titers of C components tested in pooled serum samples from the 8 species of clinically healthy domestic animals are presented.
使用市售的绵羊红细胞细胞中间体以及补体（C）系统功能纯的豚鼠和人补体成分，对8种家畜血清中的总补体（C）及其成分进行了检测。检测按照生产商推荐的检测人C成分的方法进行。在肉食动物（狗和猫）和杂食动物（猪）中检测到了晚期作用成分（C6至C9）和C1。在大型草食动物（牛、马、羊和山羊）中，C4、C2、C3和C5检测不到或效价很低，这表明与人类或豚鼠的C成分相比存在重大差异。猪血清中含有一种抑制物质，在血清稀释至1:100时会干扰C2和晚期作用成分的检测。在鸡血清中检测到了除C2以外的所有成分。C4、C2、C3和C5的结合或激活（或两者）比其他成分的结合或激活（或两者）更具物种特异性。未检测到抗体与C1之间的物种特异性要求。在牛、羊、马和羊血清中检测到了C1灭活剂。列出了在8种临床健康家畜的混合血清样本中检测的C成分的CH50（50%溶血）效价。
