Curry F E
Am J Physiol. 1981 Apr;240(4):H597-605. doi: 10.1152/ajpheart.1981.240.4.H597.
The mechanisms of transport of water and solutes across both hydrophilic and lipophilic pathways in the capillary wall were investigated. Paired measurements of the osmotic reflection and solute permeability coefficients of a lipid soluble solute (antipyrine MW 188 or aminopyrine 231) and a hydrophilic solute (glucose 180) were made on individually perfused capillaries of frog mesentery at temperatures between 5 and 25 degrees C. Below 15 degrees C, the lipophilic pathway accounts for less than 10% of the flux of antipyrine across the capillary wall, whereas at 25 degrees C the lipophilic pathway accounts of 50% of the flux. These results conform to the hypothesis that water and both hydrophilic and lipophilic solutes share an extracellular pathway, but water and lipids cross the cell by entirely separate routes. Both the activation energy for antipyrine and aminopyrine diffusion in the lipophilic pathway (25.9 kcal/mol) and the estimated cell membrane-water partition coefficients of the solutes are similar to values found for mammalian erythrocytes. Lateral diffusion in the cell membranes would account for the lipophilic pathway permeability just as well as diffusion across the cell membranes.
研究了水和溶质在毛细血管壁中通过亲水和亲脂途径的转运机制。在5至25摄氏度的温度下，对青蛙肠系膜单个灌注毛细血管中脂溶性溶质（安替比林分子量188或氨基比林231）和亲水性溶质（葡萄糖180）的渗透反射系数和溶质渗透系数进行了配对测量。在15摄氏度以下，亲脂途径占安替比林穿过毛细血管壁通量的不到10%，而在25摄氏度时，亲脂途径占通量的50%。这些结果符合以下假设：水以及亲水性和亲脂性溶质共享细胞外途径，但水和脂质通过完全不同的途径穿过细胞。亲脂途径中安替比林和氨基比林扩散的活化能（25.9千卡/摩尔）以及溶质的估计细胞膜-水分配系数与哺乳动物红细胞的值相似。细胞膜中的横向扩散与跨细胞膜的扩散一样，可以解释亲脂途径的通透性。
