Hardy K J, Alspaugh M A
Cell Differ. 1981 Mar;10(2):93-9. doi: 10.1016/0045-6039(81)90017-8.
An antibody was produced in rabbits using human dehistonized chromatin from T lymphocytes in continuous cell culture (Jm) as immunogen. The antibody was characterized by a micro-complement fixation (CF) method against chromatin from Jm cells as well as other sources. The antibody reacted only with chromatin of transformed lymphocytes, obtained either in vitro from tissue culture, or in vivo specifically from chronic lymphocytic leukemia (CLL). This antibody did not react with chromatin of normal human or non-human lymphocytes, or with chromatin of two other related non-lymphoid human tumor types, namely chronic myelocytic leukemia (CML) and HeLa cells in continuous culture. The data suggest that there are common antigens specific to the nucleus of transformed human lymphocytes which may be related to the maintenance and/or development of this particular state of differentiation.
以连续细胞培养（Jm）中T淋巴细胞的人去组蛋白染色质为免疫原，在兔体内产生了一种抗体。该抗体通过微量补体结合（CF）法针对Jm细胞以及其他来源的染色质进行了表征。该抗体仅与转化淋巴细胞的染色质发生反应，这些转化淋巴细胞要么是从组织培养体外获得的，要么是在体内从慢性淋巴细胞白血病（CLL）特异性获得的。这种抗体不与正常人或非人类淋巴细胞的染色质反应，也不与另外两种相关的非淋巴细胞性人类肿瘤类型（即慢性粒细胞白血病（CML）和连续培养的HeLa细胞）的染色质反应。数据表明，存在转化人淋巴细胞细胞核特有的共同抗原，这些抗原可能与这种特定分化状态的维持和/或发展有关。
