Lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71. II. Isolation of the receptor.

The lymphoid cell surface receptor for Moloney leukemia virus envelope glycoprotein gp71 was isolated by using anti-gp71 antibodies to immunoprecipitate the receptor-gp71 complex from detergent extract of radiolabeled murine thymus cells. Upon sodium dodecyl sulfate gel electrophoresis, a single molecule with an apparent m.w. of 190,000 was identified as the putative gp71-receptor. Analysis in nonreducing conditions indicated that the receptor may be composed of at least 2 subunits of 190,000 daltons.