Ben-Sasson S Z, Kagan J
J Immunol Methods. 1981;41(3):321-31. doi: 10.1016/0022-1759(81)90194-0.
Various macrophage-containing preparations were tested for their ability to increase the antigen-specific proliferative response of murine T-lymphocytes. The preparations examined included: peritoneal exudate cells (PEC) from mice injected with mineral oil or thioglycolate; fresh bone-marrow cells; bone marrow cells grown in culture for up to 11 days; normal spleen cells, and spleen cells from mice injected with mineral oil. The best proliferative response was obtained when the lymphocytes were supplemented with 30% spleen cells from mice injected with mineral oil. When spleen cells from mineral oil injected mice are compared with those of spleen cells from normal mice, it is evident that mineral oil given i.p. activates the spleen macrophages. Although the number and percentage of macrophages in the spleen does not increase following mineral oil injection, the activities of some of their enzymes (acid phosphatase and beta-glucuronidase) increase while others do not change (Cathepsin D and lysozyme). Furthermore, the Fc-dependent phagocytic activity of spleen macrophages and their spreading on plastic culture dishes is increased after mineral oil treatment. We conclude that the activation of spleen macrophages caused by an i.p. injection of mineral oil also induces the changes in their antigen-presenting apparatus. Consequently, macrophages from spleens of mineral oil-injected mice are most suitable cell preparations for antigen presentation to T-lymphocytes.
对各种含巨噬细胞的制剂进行了测试，以评估它们增强小鼠T淋巴细胞抗原特异性增殖反应的能力。所检测的制剂包括：注射了矿物油或巯基乙酸盐的小鼠的腹腔渗出细胞（PEC）；新鲜骨髓细胞；在培养中生长长达11天的骨髓细胞；正常脾细胞，以及注射了矿物油的小鼠的脾细胞。当淋巴细胞与30%注射了矿物油的小鼠的脾细胞一起培养时，获得了最佳的增殖反应。将注射了矿物油的小鼠的脾细胞与正常小鼠的脾细胞进行比较时，很明显腹腔注射矿物油会激活脾巨噬细胞。虽然注射矿物油后脾脏中巨噬细胞的数量和百分比没有增加，但其一些酶（酸性磷酸酶和β-葡萄糖醛酸酶）的活性增加，而其他酶（组织蛋白酶D和溶菌酶）则没有变化。此外，矿物油处理后，脾巨噬细胞的Fc依赖性吞噬活性及其在塑料培养皿上的铺展能力增强。我们得出结论，腹腔注射矿物油引起的脾巨噬细胞激活也会诱导其抗原呈递装置的变化。因此，来自注射了矿物油的小鼠脾脏的巨噬细胞是用于向T淋巴细胞呈递抗原的最合适细胞制剂。
