Knop J, Malorny U, Macher E
Cell Immunol. 1984 Oct 15;88(2):411-20. doi: 10.1016/0008-8749(84)90174-6.
The role of various subpopulations of antigen-presenting macrophages in the induction of T-lymphocyte subpopulations has been difficult to study in the past. We have used an in vitro system of bone marrow cell culture both to induce T-effector (TDH) and T-suppressor (Ts) cells active in delayed-type hypersensitivity. Bone marrow-derived macrophages (BM-MA) grown in Teflon bag cultures were allowed to attach to culture dishes and were pulse-labeled with 2,4-dinitrobenzene sulfonate (DNBSO3). Spleen cell lymphocytes from nonsensitized BALB/c mice were cocultured with antigen-pulsed or control BM-MA for 3 days. The lymphocytes were harvested, and injected iv into BALB/c mice which were challenged within 1 hr after injection by painting the right ear with 2,4-dinitrofluorobenzene (DNFB, effector test) or sensitized with DNFB on 2 days following iv injection of the cells and challenged 5 days later (suppressor test). Ear swelling was measured 24 hr later to assess the effector or suppressor function of the in vitro educated lymphocytes. BM-MA grown for 5 days (BM-MA 5) in L-cell conditioned medium induced only TDH cells (Thy 1+, Lyt 1+2-) whereas BM-MA grown for 10 days in conditioned medium induced only Ts cells (Thy 1+, Lyt 1-2+). In both cases, induced TDH and Ts cells were antigen specific. Functionally, induced Ts cells suppressed the afferent limb of the delayed response. When DNP-BM-MA 5 and DNP-BM-MA 10 were used to induce TDH or Ts cells in vivo by subcutaneous or intravenous injection respectively, only BM-MA 5 were able to sensitize recipient mice. Both 5- and 10-day macrophage populations induced Ts cells in vivo. Functionally, these Ts cells appeared to act on the efferent limb of the delayed reaction. We conclude that different populations of antigen-presenting macrophages can preferentially induce TDH or Ts cells, perhaps depending on antigen presentation in association with class II antigens or on the functional state of the antigen-presenting cell.
过去，抗原呈递巨噬细胞的各种亚群在诱导T淋巴细胞亚群方面的作用一直难以研究。我们使用了一种骨髓细胞培养的体外系统来诱导在迟发型超敏反应中具有活性的T效应细胞（TDH）和T抑制细胞（Ts）。在聚四氟乙烯袋培养物中生长的骨髓来源巨噬细胞（BM-MA）被允许附着于培养皿，并使用2,4-二硝基苯磺酸盐（DNBSO3）进行脉冲标记。将来自未致敏BALB/c小鼠的脾细胞淋巴细胞与经抗原脉冲处理或对照的BM-MA共培养3天。收获淋巴细胞，并静脉注射到BALB/c小鼠体内，在注射后1小时内通过用2,4-二硝基氟苯（DNFB）涂抹右耳进行攻击（效应测试），或者在静脉注射细胞后2天用DNFB致敏，并在5天后进行攻击（抑制测试）。24小时后测量耳部肿胀，以评估体外培养的淋巴细胞的效应或抑制功能。在L细胞条件培养基中培养5天的BM-MA（BM-MA 5）仅诱导TDH细胞（Thy 1 +，Lyt 1 + 2 -），而在条件培养基中培养10天的BM-MA仅诱导Ts细胞（Thy 1 +，Lyt 1 - 2 +）。在这两种情况下，诱导的TDH和Ts细胞都是抗原特异性的。在功能上，诱导的Ts细胞抑制迟发型反应的传入支。当分别通过皮下或静脉注射使用DNP-BM-MA 5和DNP-BM-MA 10在体内诱导TDH或Ts细胞时，只有BM-MA 5能够使受体小鼠致敏。5天和10天的巨噬细胞群体在体内均诱导Ts细胞。在功能上，这些Ts细胞似乎作用于迟发型反应的传出支。我们得出结论，不同群体的抗原呈递巨噬细胞可以优先诱导TDH或Ts细胞，这可能取决于与II类抗原相关的抗原呈递或抗原呈递细胞的功能状态。
