以LCL细胞作为饲养层，在TCGF中对“克隆的”人血小板（PLT）细胞进行长期维持培养。
Long-term maintenance of "cloned" human PLT cells in TCGF with LCL cells as a feeder layer.
作者信息
Hank J A, Inouye H, Guy L A, Alter B J, Bach F H
出版信息
J Supramol Struct. 1980;13(4):525-32. doi: 10.1002/jss.400130411.
PMID:6971962
Abstract
The long-term maintenance of T cells "cloned" by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 X 10(12) cells from a "clone" seeded at one cell per well. Some of the clones tested express primed LD-typing activity.
摘要
通过使用经照射的自体淋巴母细胞系（LCL）细胞以及经照射的最初使T细胞致敏个体的LCL细胞，增强了通过在TCGF中有限稀释“克隆”的T细胞的长期维持。从每孔接种一个细胞的“克隆”中有可能获得超过1×10¹²个细胞。一些测试的克隆表达致敏的LD分型活性。
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