Boylston A W, Anderson R L, Haworth C A
Clin Exp Immunol. 1981 Feb;43(2):329-35.
Human T lymphocytes (CCTC) have been maintained in continuous culture by a new method. The ability of CCTC to produce three types of T cell response has been determined and compared to the published responses of T cells grown in 'T cell growth factor' (TCGF) medium. Unlike TCGF cells, CCTC will not give proliferative responses when stimulated with PHA, Con A, or allogeneic lymphocytes even when supplemented with autologous PBL as a source of accessory cells. Instead, CCTC are potent inhibitors of the proliferative response of normal cells in MLC. This suppressive activity is not specific for histocompatible cells. Finally, CCTC express cytotoxic activity to a number of human lymphoid cell lines and this activity appears to be different from the polyclonal cytotoxicity reported for TCGF-maintained cells. Our method of long-term T cell culture therefore appears to grow cells with different properties from TCGF cells.
人类T淋巴细胞（CCTC）已通过一种新方法进行连续培养。已确定CCTC产生三种类型T细胞反应的能力，并与在“T细胞生长因子”（TCGF）培养基中生长的T细胞已发表的反应进行了比较。与TCGF细胞不同，即使补充自体PBL作为辅助细胞来源，CCTC在用PHA、Con A或同种异体淋巴细胞刺激时也不会产生增殖反应。相反，CCTC是混合淋巴细胞培养中正常细胞增殖反应的有效抑制剂。这种抑制活性对组织相容性细胞没有特异性。最后，CCTC对多种人类淋巴细胞系表现出细胞毒性活性，并且这种活性似乎与报道的TCGF培养细胞的多克隆细胞毒性不同。因此，我们的长期T细胞培养方法似乎培养出了具有与TCGF细胞不同特性的细胞。
