Aso K, Kondo S, Amano M
Curr Probl Dermatol. 1980;10:83-9. doi: 10.1159/000396283.
Agglutinated platelets are known to release a factor that stimulates the growth of arterial smooth muscle cells or dermal fibroblasts. The present study was designed to see the effect of this platelet factor on epidermal cells. The growth of cultured epidermal cells and dermal fibroblasts obtained from guinea pig ear skin was observed using 10% platelet-rich serum or platelet-poor serum in the culture medium. These sera were prepared from fresh human blood according to the method described by Ross. The cell growth was checked by counting the number of cells in culture or by the uptake of labeled thymidine by the cells. The platelet-rich serum significantly stimulated the growth of epidermal cells as well as fibroblasts as compared with the platelet-poor serum. This implies that platelet factor released after injury to cutaneous vessels in trauma or inflammation plays an important role in initiating proliferation of epidermal cells and dermal fibroblasts.
已知凝集的血小板会释放一种刺激动脉平滑肌细胞或真皮成纤维细胞生长的因子。本研究旨在观察这种血小板因子对表皮细胞的作用。使用培养基中10%的富血小板血清或贫血小板血清，观察从豚鼠耳皮肤获得的培养表皮细胞和真皮成纤维细胞的生长情况。这些血清是根据罗斯描述的方法从新鲜人血中制备的。通过计算培养物中的细胞数量或细胞对标记胸苷的摄取来检查细胞生长。与贫血小板血清相比，富血小板血清显著刺激了表皮细胞和成纤维细胞的生长。这意味着在创伤或炎症中皮肤血管损伤后释放的血小板因子在启动表皮细胞和真皮成纤维细胞的增殖中起重要作用。
