Falchuk Z M, Barnhard E, Machado I
Gut. 1981 Apr;22(4):290-4. doi: 10.1136/gut.22.4.290.
We isolated lymphocytes from the lamina propria of colon from 19 patients with inflammatory bowel disease, colon cancer, and certain benign conditions to determine: (1) if these lymphocytes could mediate mitogen-induced (MICC) and spontaneous cell-mediated cytotoxicity (SCMC), and (2) if there were any differences in cytotoxic effectiveness which could relate to the underlying disease. We found that lamina propria lymphocytes functioned well in MICC reactions with phytohaemagglutinin, but not concanavalin A as the inducing mitogen (specific lysis 28 5% vs 5 3%). Lamina propria lymphocytes did not mediate SCMC (specific lysis 0.3%). Neither the presence of inflammation not the underlying disease of the patient influenced the cytotoxic activity. Peripheral blood lymphocytes from normal subjects and patients performed well in MICC assay with both phytohaemagglutinin and concanvalin A as the inducing mitogen and were equally effective in SCMC reactions.
我们从19例患有炎症性肠病、结肠癌和某些良性疾病的患者的结肠固有层中分离淋巴细胞，以确定：（1）这些淋巴细胞是否能介导丝裂原诱导的细胞毒性（MICC）和自发细胞介导的细胞毒性（SCMC），以及（2）细胞毒性效力是否存在与潜在疾病相关的差异。我们发现，固有层淋巴细胞在以植物血凝素作为诱导丝裂原的MICC反应中功能良好，但以刀豆球蛋白A作为诱导丝裂原时则不然（特异性裂解率分别为28.5%和5.3%）。固有层淋巴细胞不介导SCMC（特异性裂解率为0.3%）。炎症的存在和患者的潜在疾病均不影响细胞毒性活性。正常受试者和患者的外周血淋巴细胞在以植物血凝素和刀豆球蛋白A作为诱导丝裂原的MICC试验中表现良好，并且在SCMC反应中同样有效。
