Glick M E, Falchuk Z M
Gut. 1981 Feb;22(2):120-5. doi: 10.1136/gut.22.2.120.
Dinitrochlorobenzene-induced colitis in guinea-pigs may be immunologically mediated: animals must be presensitised to dinitrochlorobenzene to develop colitis, sensitivity can be passively transferred by lymphocytes and the injury can be mitigated by immunosuppression. In this study, we examined lamina propria lymphocytes isolated from colons of animals with dinitrochlorobenzene-induced colitis, and appropriate controls. Lamina propria lymphocytes from colitis animals have a greater percentage of rabbit erythrocyte-rosetting cells (T cells) (20.1 +/- 3.0 vs 2.3 +/- 0.8, p less than .01) and a greater capacity to mediate mitogen-induced cellular cytotoxicity with phytohaemagglutinin than lamina propria lymphocytes from normal colon (% specific cytoxicity = 29.4 +/- 8.7 vs 5.0 +/- 4.5, P less than .005). There was no difference in the percentage of rosetting cells or cytotoxicity index of spleen or mesenteric lymph node lymphocytes between the colitis animals and controls. These data suggest that there are changes in the distribution and functional characteristics of lamina propria lymphocytes which correlate with mucosal cell injury in the dinitrochlorobenzene-colitis model.
动物必须预先对二硝基氯苯致敏才能发生结肠炎，敏感性可通过淋巴细胞被动转移，且损伤可通过免疫抑制减轻。在本研究中，我们检测了从患有二硝基氯苯诱导的结肠炎的动物结肠及适当对照中分离出的固有层淋巴细胞。来自结肠炎动物的固有层淋巴细胞中，兔红细胞花环形成细胞（T细胞）的百分比更高（20.1±3.0对2.3±0.8，p＜0.01），并且与来自正常结肠的固有层淋巴细胞相比，用植物血凝素介导丝裂原诱导的细胞毒性的能力更强（特异性细胞毒性百分比=29.4±8.7对5.0±4.5，P＜0.005）。结肠炎动物与对照之间，脾脏或肠系膜淋巴结淋巴细胞的花环形成细胞百分比或细胞毒性指数没有差异。这些数据表明，固有层淋巴细胞的分布和功能特性发生了变化，这与二硝基氯苯-结肠炎模型中的黏膜细胞损伤相关。
