Padrón R, Mateu L
J Neurosci Res. 1981;6(2):251-60. doi: 10.1002/jnr.490060212.
The x-ray diffraction pattern of myelin membranes from a frog sciatic nerve was recorded in vivo at 13 A resolution using a new experimental technique recently developed in our laboratory. The electron density profile was determined for the myelin membranes first in vivo and then, after elimination of the intraneural blood microcirculation, by transection of the nerve. The small structural differences observed between these conditions were statistically nonsignificant (at 95% confidence level). In addition, our results show that the stacking disorder that is present in the myelin membranes of freshly dissected frog sciatics is also characteristic of the myelin sheath in vivo.
利用我们实验室最近开发的一种新实验技术，在体内以13埃的分辨率记录了青蛙坐骨神经髓鞘膜的X射线衍射图谱。首先在体内确定髓鞘膜的电子密度分布，然后在切断神经以消除神经内血液微循环后再次确定。在这些条件下观察到的微小结构差异在统计学上不显著（置信水平为95%）。此外，我们的结果表明，新鲜解剖的青蛙坐骨神经髓鞘膜中存在的堆积紊乱也是体内髓鞘的特征。
