Abuaf N, Leynadier F, Luce H, Dry J
Clin Exp Immunol. 1981 Apr;44(1):145-51.
In stimulated cultures, a proportion of human T lymphocytes form multilayer rosettes with sheep red blood cells (SRBC) and they can be easily separated from common E rosettes by velocity sedimentation. We have developed a simplified method for harvesting multilayer rosettes and quantifying them. Practical applications of this technique are discussed.
在受刺激的培养物中,一部分人T淋巴细胞可与绵羊红细胞(SRBC)形成多层玫瑰花结,并且通过速度沉降法可轻松将它们与普通E玫瑰花结分离。我们已开发出一种用于收获多层玫瑰花结并对其进行定量的简化方法。本文讨论了该技术的实际应用。