[豚鼠T淋巴细胞和B淋巴细胞计数的测定]
[Determination of T- and B-lymphocyte counts in guinea pigs].
作者信息
Shvartsman M Ia, Kashkin A P
出版信息
Biull Eksp Biol Med. 1982 Sep;94(9):85-7.
T lymphocytes of guinea-pigs were found to form spontaneous E-rosettes with rabbit red blood cells. The lymphocyte count constituted 32% of blood lymphocytes, 41% of lymph node cells. Meanwhile no T lymphocytes were detected in the bone marrow. The T lymphocyte subpopulation capable of forming "early" rosettes was demonstrated. B lymphocytes formed EAC-rosettes with sheep red blood cells. The B lymphocyte count in the blood, lymph nodes and bone marrow amounted to 10, 18 and 5%, respectively. The effects of incubation conditions, centrifugation, antibody types, and complement concentration, etc. on E- and EAC-rosette formation were defined.
发现豚鼠的T淋巴细胞能与兔红细胞形成自发E花环。淋巴细胞计数在血液淋巴细胞中占32%，在淋巴结细胞中占41%。同时，在骨髓中未检测到T淋巴细胞。证明了能够形成“早期”花环的T淋巴细胞亚群。B淋巴细胞与绵羊红细胞形成EAC花环。血液、淋巴结和骨髓中的B淋巴细胞计数分别为10%、18%和5%。确定了孵育条件、离心、抗体类型和补体浓度等对E花环和EAC花环形成的影响。
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