Burrow H M, Bird C C, Warren J V, Steel C M, Barrett I D, Panesar N S
Diagn Histopathol. 1981 Apr-Jun;4(2):175-88.
Using various genotypic and phenotypic markers 20 human lymphoid cell lines have been classified as lymphoblastoid, leukaemia or lymphoma subtypes. Each cell line type exhibited characteristic morphological and behavioural properties in suspension culture. Whilst most lymphoblastoid and lymphoma cell lines manifested B-cell phenotypes and contained Epstein Barr virus (EBV) genome, leukaemia lines demonstrated T-cell markers and lacked EBV genome. Individual cell lines demonstrated unique isoenzyme profiles for the seven polymorphic enzymes studied without subtype specificity. None of the cell lines studied was entirely homogeneous although lymphoblastoid lines contained only a minor subpopulation of other cell line types. The mixed population of cells indicate the need for caution in the use of these cell lines as in vitro models of lymphoid cancer and suggests further refinement of classification methods is required. Incubation of different cell line types with prednisolone for 48-168 h revealed most were highly resistant to cytolethal and cytostatic effects of glucocorticoids in vitro. Suprapharmacological doses of steroid (10(-3) M) were required in most instances before significant cytolethal responses occurred. Only one lymphoblastoid, one lymphoma and two leukaemia lines responded to pharmacological doses (10(-5)-10(-6) M) of prednisolone.
利用各种基因型和表型标记，已将20种人类淋巴细胞系分为淋巴母细胞样、白血病或淋巴瘤亚型。每种细胞系类型在悬浮培养中表现出特征性的形态和行为特性。虽然大多数淋巴母细胞样和淋巴瘤细胞系表现出B细胞表型并含有爱泼斯坦-巴尔病毒（EBV）基因组，但白血病细胞系显示T细胞标记且缺乏EBV基因组。各个细胞系在所研究的七种多态性酶方面表现出独特的同工酶谱，无亚型特异性。尽管淋巴母细胞样细胞系仅含有少量其他细胞系类型的亚群，但所研究的细胞系均无完全均一的情况。细胞的混合群体表明在将这些细胞系用作淋巴癌的体外模型时需谨慎，并提示需要进一步完善分类方法。将不同细胞系类型与泼尼松龙孵育48 - 168小时发现，大多数细胞系在体外对糖皮质激素的细胞致死和细胞抑制作用具有高度抗性。在大多数情况下，在出现明显的细胞致死反应之前需要超药理剂量的类固醇（10⁻³ M）。只有一个淋巴母细胞样细胞系、一个淋巴瘤细胞系和两个白血病细胞系对药理剂量（10⁻⁵ - 至10⁻⁶ M）的泼尼松龙有反应。
