Bird C C, Waddell A W, Robertson A M, Currie A R, Steel C M, Evans J
Br J Cancer. 1975 Dec;32(6):700-7. doi: 10.1038/bjc.1975.281.
The cytolethal response to treatment with prednisolone was investigated in vitro in eight human lymphoblastoid cell lines containing varying concentrations of specific cytoplasmic glucocorticoid receptors. A similar response was observed in seven of the lines irrespective of their concentration of cytoplasmic receptors and pharmacological doses of steroid, well above those required to saturate receptors in cell-free extracts, were required for a massive lethal response. One cell line derived from Burkitt's lymphoma was refractory to lethal effects even with pharmacological doses of steroid. A similar unresponsiveness to the cytolethal effect of prednisolone in vitro was observed in fresh lymphoblasts derived from patients with acute lymphoblastic leukaemia despite evidence of a satisfactory clinical response to therapy which included steroid. The resistance of human lymphoblastoid cells to treatment with glucocorticoids in vitro may result from a defect in activation subsequent to the binding of steroid to cytoplasmic receptors.
在体外研究了泼尼松龙处理对八种含有不同浓度特异性细胞质糖皮质激素受体的人淋巴母细胞系的细胞致死反应。在七个细胞系中观察到了类似的反应,无论它们细胞质受体的浓度如何,都需要高于使无细胞提取物中的受体饱和所需剂量的药理剂量的类固醇才能产生大规模致死反应。一种源自伯基特淋巴瘤的细胞系即使使用药理剂量的类固醇也对致死效应具有抗性。尽管有证据表明包括类固醇在内的治疗有令人满意的临床反应,但在急性淋巴细胞白血病患者的新鲜淋巴母细胞中也观察到对泼尼松龙体外细胞致死效应的类似无反应性。人淋巴母细胞系在体外对糖皮质激素处理的抗性可能是由于类固醇与细胞质受体结合后激活缺陷所致。