Pujol R, Sans A, Calas A
Eur Neurol. 1981;20(3):157-61. doi: 10.1159/000115225.
Light and high resolution radioautography were performed on cat and guinea pig inner ear after an in vivo administration of tritiated deoxyglucose and conventional histological treatments. In the guinea pig cochlea a diffuse radioautographic reaction product appeared on all the neurosensory and surrounding structures, with a more intense labelling of stria vascularis and Reissner's membrane. In the cat vestibular organ a diffuse reaction was also noted, with an intense clear-cut labelling of some dark cells at the base of the lateral crista and on some cells limiting the endolymphatic space, opposite the utricular macula. At the EM level, the silver grain distribution preferentially appeared to be localized on the cytoplasmic glycogen granules. In addition, some sensory cells were densely marked, the silver grains neither occurring on the nucleus nor on the surrounding nerve calyx, and rarely upon the mitochondria. These data indicate that using classical histological treatments, it is possible to retain a part of the radioactive molecules around the injection site. The possible physiological meaning of the selective labeling of some cellular and subcellular compartments is discussed.
在给猫和豚鼠体内注射氚标记的脱氧葡萄糖并进行常规组织学处理后，对其内耳进行了光镜和高分辨率放射自显影检查。在豚鼠耳蜗中，所有神经感觉结构和周围结构均出现弥漫性放射自显影反应产物，血管纹和Reissner膜的标记更为强烈。在猫的前庭器官中也观察到弥漫性反应，在外侧嵴底部的一些暗细胞以及与椭圆囊斑相对的限制内淋巴间隙的一些细胞上有强烈且清晰的标记。在电子显微镜水平上，银颗粒分布似乎优先定位于细胞质糖原颗粒上。此外，一些感觉细胞被密集标记，银颗粒既不位于细胞核上，也不位于周围的神经杯上，很少出现在线粒体上。这些数据表明，使用经典组织学处理方法，可以在注射部位周围保留一部分放射性分子。文中还讨论了某些细胞和亚细胞区室选择性标记的可能生理意义。
