Zipori D, Sasson T, Frenkel A
Exp Hematol. 1981 Jul;9(6):656-63.
Colony stimulating factor (CSF) was incapable of inducing the formation of granulocyte/monocyte (G/M) colonies in the presence of bone marrow-derived adherent cells. To test the possibility that interactions between adherent cells and myeloid progenitors are mediated via glycoproteins, we added a variety of sugars to methyl-cellulose cultures of BALB/c mouse bone marrow cells, in the presence of syngeneic bone marrow adherent cells. We found that a number of free sugars, as well as certain glycosides, relieved the inhibition of G/M colony formation exerted by the adherent cells. The effect of these monosaccharides was neither due to osmotic changes nor to their toxicity to the adherent cells. It is therefore concluded that glycoprotein or glycolipid factors may be involved in the interactions between myeloid progenitors and stromal cells.
在存在骨髓来源的贴壁细胞的情况下，集落刺激因子（CSF）无法诱导粒细胞/单核细胞（G/M）集落的形成。为了测试贴壁细胞与髓系祖细胞之间的相互作用是否通过糖蛋白介导，我们在同基因骨髓贴壁细胞存在的情况下，向BALB/c小鼠骨髓细胞的甲基纤维素培养物中添加了多种糖类。我们发现，许多游离糖以及某些糖苷可缓解贴壁细胞对G/M集落形成的抑制作用。这些单糖的作用既不是由于渗透压变化，也不是由于它们对贴壁细胞的毒性。因此得出结论，糖蛋白或糖脂因子可能参与了髓系祖细胞与基质细胞之间的相互作用。
