Zipori D, Sasson T
Exp Hematol. 1981 Jul;9(6):663-74.
The inhibition of myeloid colony formation exerted by adherent bone marrow cells could be relieved by various non-metabolized methylglycosides and also by the free sugar D-glucose. The formation of colonies in the presence of the latter had, however, a number of distinct features. To test whether the effect of D-glucose was due to its metabolism, we tried to mimic the glucose effect by pyruvate and lactate. These could not induce colony formation in the presence of bone marrow stromal cells. Glucose-induced colonies could also form on top of agar overlayering the adherent cells. It therefore appears that stromal cells produce a stimulator of myelopoiesis which is glucose-dependent. This factor is capable of partially overcoming the activity of the inhibitor concomitantly produced by stromal cells.
贴壁骨髓细胞对髓系集落形成的抑制作用可被多种非代谢性甲基糖苷以及游离糖D-葡萄糖解除。然而，在后者存在的情况下集落的形成具有一些明显的特征。为了测试D-葡萄糖的作用是否归因于其代谢，我们试图用丙酮酸和乳酸模拟葡萄糖的作用。在骨髓基质细胞存在的情况下，这些物质不能诱导集落形成。葡萄糖诱导的集落也可以在覆盖贴壁细胞的琼脂上形成。因此，似乎基质细胞产生了一种葡萄糖依赖性的骨髓生成刺激因子。该因子能够部分克服基质细胞同时产生的抑制剂的活性。
