细胞溶解性T淋巴细胞功能独立于生长阶段和有丝分裂周期中的位置。
Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle.
作者信息
Sekaly R P, MacDonald H R, Zaech P, Glasebrook A L, Cerottini J C
出版信息
J Exp Med. 1981 Aug 1;154(2):575-80. doi: 10.1084/jem.154.2.575.
We have investigated mitotic cell cycle and growth phase regulation of homogeneous cytolytic T lymphocytes (CTL). Two independently derived CTL clones were stained with the DNA-binding dye Hoechst 33342, sorted in a fluorescence-activated cell sorter according to their position in the cell cycle, and then assayed for specific lytic activity using a short-term (30 min) (51)Cr release assay. Results show that lytic activity remained unchanged throughout the cell cycle. Furthermore, there was no significant difference in the lytic activity of CTL clones growing exponentially or arrested in a plateau phase. These results demonstrate that T cell-mediated cytolysis is independent of growth phase and position in the cell cycle.
我们研究了同源细胞毒性T淋巴细胞（CTL）的有丝分裂细胞周期和生长阶段调控。用DNA结合染料Hoechst 33342对两个独立衍生的CTL克隆进行染色，根据它们在细胞周期中的位置在荧光激活细胞分选仪中进行分选，然后使用短期（30分钟）（51）Cr释放试验检测其特异性裂解活性。结果表明，在整个细胞周期中裂解活性保持不变。此外，处于指数生长期或停滞在平台期的CTL克隆的裂解活性没有显著差异。这些结果表明，T细胞介导的细胞溶解与生长阶段和细胞周期位置无关。
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