Novokhatskiĭ A S, Labzo S S, Melik-Andreasian G G, Tsareva A A, Mikhaĭlova G R
Vopr Virusol. 1981 Mar-Apr(2):153-60.
The results of virological investigations of alphavirus persistence in human B-cell lines Raji and L-101 are presented. The formation of persisting infection was shown to depend both on the cell line and on the virus type. Productive persistent infection of Raji cells with Venezuelan equine encephalitis virus was followed for 11 months. The presence of the virus was confirmed by electron microscopic and immunofluorescent examinations. Infectious virus production varied from 0.001 to dozens PFU/cell, and the content of viable cells from 100,000 to 300,000 in 1 ml of the culture fluid. Virus infectivity in the culture medium varied within the range of 5-7 lg PFU/ml. Human lymphoblastoid cells Raji persistently infected with Venezuelan equine encephalitis virus were examined cytologically, karyologically, and electron microscopically. The long-term presence of the virus resulted in profound alterations in the cell population. Morphology of the cells and processes of division were changed, the mitotic index decreased, the rate of 3H-thymidine incorporation into cellular DNA increased. The mechanisms of persistence are discussed.
本文展示了对甲病毒在人B细胞系Raji和L - 101中持续存在情况的病毒学研究结果。研究表明，持续感染的形成既取决于细胞系，也取决于病毒类型。用委内瑞拉马脑炎病毒对Raji细胞进行了11个月的生产性持续感染研究。通过电子显微镜和免疫荧光检查确认了病毒的存在。感染性病毒产量为每细胞0.001至数十个空斑形成单位（PFU），每毫升培养液中活细胞含量为100,000至300,000个。培养基中病毒感染性在5 - 7 log PFU/ml范围内变化。对持续感染委内瑞拉马脑炎病毒的人淋巴母细胞系Raji进行了细胞学、染色体学和电子显微镜检查。病毒的长期存在导致细胞群体发生深刻变化。细胞形态和分裂过程发生改变，有丝分裂指数降低，3H - 胸腺嘧啶核苷掺入细胞DNA的速率增加。文中讨论了持续存在的机制。
